PCR 滤光片

益瑞电是滤光片解决方案的领导者,现在提供专为实时 PCR(聚合酶链反应)应用设计的超高性能滤光片。 我们的标准滤光片解决方案与常见的激发源和荧光团兼容,但我们专注于定制滤光片解决方案。 Ultra PCR 荧光滤光片组设计用于在 LED 光源照射时提供实时 PCR 样品中所含探针的高信噪比。

益瑞电的发射、激发和二向色滤光片为荧光仪器用户和原始设备制造商提供更少背景的更多信号。

Dichroic filters

  • Mechanical:
    • – Filters: 12 x 8 mm, 12 x 17 mm
    • – Filter Thickness 1.1 mm
    • – Clear aperture: > 90%
    • – Substrate: Fused Silica – Scratch/Dig: 60/40
  • Optical:
    • – Tx > 95% average on passbands
    • – Reflection > 97% over blocking range
    • – AOI: 45 +/- 2 degrees; CHA: 5 degrees
    • – Steep slope: < 1% (90%T – 10%T)
FluorochromeFilter Name
FAM ChannelPCR 505nm DLP
HEX ChannelPCR 550nm DLP
ROX ChannelPCR 590nm DLP
Cy5 ChannelPCR 647nm DLP
Cy5.5 ChannelPCR 700nm DLP
 

HEX/VIC/JOE Channel:
EX Filter: 530-20nm; Dichroic: 550DLP; EM Filter: 565-22nm

 

 

Excitation and Emission Filters

 

  • Mechanical:
    • – Filters: 8 x 8 mm, 12 x 12 mm, 25 mm diam.
    • – Filter Thickness 1.1 mm
    • – Clear aperture: > 90%
    • – Substrate: Fused Silica – Scratch/Dig: 60/40
  • Optical:
    • – Tx > 95% average on passbands
    • – Average blocking > OD6 from 350-850 nm outside of passband
    • – Steep slope: < 0.5% (50% Tmax- OD5)
    • – AOI: 0 – 5 degrees; CHA: 5 degrees
FluorochromeFilter Name
FAM Channel470-15nm EX /523-13nm EM
HEX Channel523-13nm EX/570-15nm EM
ROX Channel570-15nm EX/625-13nm EM
Cy5 Channel625-13nm EX/675-13nm EM
Cy5.5 Channel685-20nm EX/725-40nm EM

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