Iridian PCR Filters

Iridian, the leader in optical filter solutions, now offers ultra performance filters specially designed for Real Time PCR (polymerase chain reaction) applications. Our standard filter solutions are compatible with common excitation sources and fluorophores but we specialize in customized filter solutions. The ultra PCR fluorescence filter sets are designed to provide a high signal-to-noise ratio of the probes contained in Real-Time PCR samples when illuminated with a LED light source.

Iridian’s emission, excitation and dichroic filters provide more signal with less background to the fluorescence instrument users and OEMs.

Dichroic filters

  • Mechanical:
    – Filters: 12 x 8 mm, 12 x 17 mm
    – Filter Thickness 1.1 mm
    – Clear aperture: > 90%
    – Substrate: Fused Silica
    – Scratch/Dig: 60/40
  • Optical:
    – Tx > 95% average on passbands
    – Reflection > 97% over blocking range
    – AOI: 45 +/- 2 degrees; CHA: 5 degrees
    – Steep slope: < 1% (90%T – 10%T)
FluorochromeFilter Name
FAM ChannelPCR 505nm DLP
HEX ChannelPCR 550nm DLP
ROX ChannelPCR 590nm DLP
Cy5 ChannelPCR 647nm DLP
Cy5.5 ChannelPCR 700nm DLP
 

HEX/VIC/JOE Channel:
EX Filter: 530-20nm; Dichroic: 550DLP; EM Filter: 565-22nm

 

 

Excitation and Emission Filters

 

  • Mechanical:
    – Filters: 8 x 8 mm, 12 x 12 mm, 25 mm diam.
    – Filter Thickness 1.1 mm
    – Clear aperture: > 90%
    – Substrate: Fused Silica
    – Scratch/Dig: 60/40
  • Optical:
    – Tx > 95% average on passbands
    – Average blocking > OD6 from 350-850 nm
    outside of passband
    – Steep slope: < 0.5% (50% Tmax- OD5)
    – AOI: 0 – 5 degrees; CHA: 5 degrees
FluorochromeFilter Name
FAM Channel470-15nm EX /523-13nm EM
HEX Channel523-13nm EX/570-15nm EM
ROX Channel570-15nm EX/625-13nm EM
Cy5 Channel625-13nm EX/675-13nm EM
Cy5.5 Channel685-20nm EX/725-40nm EM

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Tel:  +1 (613) 741-4513
Fax: +1 (613) 741-9986

E-mail: istsales1@idexcorp.com